PMID:6291030
| Citation |
Boeke, JD and Model, P (1982) A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane. Proc. Natl. Acad. Sci. U.S.A. 79:5200-4 |
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| Abstract |
Gene III protein of bacteriophage f1 is inserted into the host cell membrane where it is assembled into phage particles. A truncated form of gene III protein, encoded by a recombinant plasmid and lacking the carboxyl terminus, does not remain in the membrane but instead appears to slip through it. Fusion of a hydrophobic "membrane anchor" from another membrane protein, the gene VIII protein, to the truncated gene III protein (by manipulation of the recombinant plasmid) restores membrane anchoring. A model for the relationship of gene III protein with the Escherichia coli membrane is discussed. |
| Links | |
| Keywords |
Cell Membrane/physiology; Cloning, Molecular; Coliphages/genetics; DNA Restriction Enzymes; Escherichia coli/genetics; Genes, Viral; Plasmids; Viral Proteins/genetics |
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