PMID:6286420
| Citation |
Brandsma, JA, Stoorvogel, J, van Sluis, CA and van de Putte, P (1982) Effect of lexA and ssb genes, present on a uvrA recombinant plasmid, on the UV survival of Escherichia coli K-12. Gene 18:77-85 |
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| Abstract |
The recombinant plasmid pJA01 contains, besides the uvrA gene, the genes lexA, ubiA and ssb. This plasmid does not fully complement a uvrA mutation in a Rec+ background. Plasmids which contain the uvrA and ssg genes, but not the lexA gene, show a higher but still only partial complementation. Full complementation achieved when the ssb gene us inactivated by insertion of Tn5. Furthermore, it appears that the presence of the ssb gene on a multicopy plasmid sensitizes wild-type cells to UV light. The effect of Ssb (single-strand DNA binding protein) overproduction on UV survival is discussed. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Carrier Proteins/genetics; DNA Transposable Elements; Escherichia coli/radiation effects; Gene Expression Regulation; Genes, Bacterial; Genetic Complementation Test; Plasmids; Serine Endopeptidases; Ultraviolet Rays |
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