PMID:6285149
| Citation |
Boidol, W, Simonis, M, Töpert, M and Siewert, G (1982) Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli. Mol. Gen. Genet. 185:510-2 |
|---|---|
| Abstract |
Restriction maps of several recombinant plasmids representing a section of the E. coli K12 chromosome 35,000 bp in size with the genes phoA, proC and phoB were prepared. The orientation of phoA and the exact position of its N-terminal end on this map were determined by identifying a subfragment which carried the phoA promoter and by determining the nucleotide sequence of a 160 bp portion of this subfragment comprising the codons for the N-terminal end of pre-alkaline phosphatase. From this DNA sequence the leader sequence of alkaline phosphatase which consists of 21 amino acids was derived. |
| Links | |
| Keywords |
Alkaline Phosphatase/genetics; Amino Acid Sequence; Base Sequence; Chromosomes, Bacterial/physiology; DNA Restriction Enzymes; DNA, Recombinant/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Plasmids |
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