PMID:6282695
| Citation |
Bagdasarian, M, Lurz, R, Rückert, B, Franklin, FC, Bagdasarian, MM, Frey, J and Timmis, KN (1981) Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene 16:237-47 |
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| Abstract |
Host-vector systems have been developed for gene cloning in the metabolically versatile bacterial genus Pseudomonas. They comprise restriction-negative host strains of Pseudomonas aeruginosa and P. putida and new cloning vectors derived from the high-copy-number, broad-host-range plasmid RSF1010, which are stably maintained in a wide range of Gram-negative bacteria. These plasmids contain EcoRI, SstI, HindIII, XmaI, XhoI, SalI, BamHI, and ClaI insertion sites. All cloning sites, except for BamHI and ClaI, are located within antibiotic-resistance genes' insertional inactivation of these genes during hybrid plasmid formation provides a readily scored phenotypic change for the rapid identification of bacterial clones carrying such hybrids. One of the new vector plasmids is a cosmid that may be used for the selective cloning of large DNA fragments by in vitro lambda packaging. An analogous series of vectors that are defective in their plasmid-mobilization function, and that exhibit a degree of biological containment comparable to that of current Escherichia coli vector plasmids, are also described. |
| Links | |
| Keywords |
Bacteriophage lambda/genetics; Base Sequence; Cloning, Molecular; Conjugation, Genetic; DNA Restriction Enzymes; DNA, Bacterial/metabolism; Genetic Vectors; Plasmids; Pseudomonas/genetics; RNA Polymerase I/metabolism |
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