PMID:6272196
| Citation |
An, G, Bendiak, DS, Mamelak, LA and Friesen, JD (1981) Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts. Nucleic Acids Res. 9:4163-72 |
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| Abstract |
We report the nucleotide sequence of the four min region of the Escherichia coli genetic map that includes the genes for ribosomal protein S2 (rpsB) and translation elongation factor EF-Ts (tsf), and the possible location of regulatory sites within this two gene operon. The data indicate that the gene order is: rpsBp-rpsB-tsf-tsft. One potential regulatory site is a 16 nucleotide sequence in the rpsB leader region encompassing the ribosome binding site and the translation initiation codon. This has a high degree of homology with nucleotides 8 through 23 on the 5' end of 165 ribosomal RNA, and might signify a sequence that is necessary for post-transcriptional control of rpsB expression. The data allow one to infer the amino acid sequences of S2 and EF-Ts. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Mapping; DNA Restriction Enzymes; Escherichia coli/genetics; Genes, Bacterial; Operon; Peptide Elongation Factors/genetics; Ribosomal Proteins/genetics |
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