PMID:6265208
| Citation |
Young, IG, Rogers, BL, Campbell, HD, Jaworowski, A and Shaw, DC (1981) Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon. Eur. J. Biochem. 116:165-70 |
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| Abstract |
The nucleotide sequence of the structural gene coding for the respiratory NADH dehydrogenase of Escherichia coli has been determined by the chain-termination method. The reading frame for the protein starts with the unusual initiation codon UUG and predicts an amino acid sequence of 434 residues (Mr = 47 304). The reading frame was confirmed by protein chemical studies including determination of the N-terminal sequence of the protein. The product made in vivo was found to have threonine as its N-terminal residue, indicating that the initiating N-formylmethionine had been removed by post-translational processing. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Codon; Cytochrome Reductases/genetics; DNA Restriction Enzymes; DNA, Bacterial/genetics; DNA, Recombinant; Escherichia coli/enzymology; Genes; Genetic Code; NADH Dehydrogenase/genetics; RNA, Messenger |
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