PMID:6263753

Citation	Covarrubias, L, Cervantes, L, Covarrubias, A, Soberón, X, Vichido, I, Blanco, A, Kupersztoch-Portnoy, YM and Bolivar, F Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328. Gene 13:25-35
Abstract	A DNA sequence essential for the R64drd11 + ColK-mediated conjugal transfer of pBR322 has been located in a 540 bp HaeIII fragment (HaeIII-2) between the vegetative origin of replication and the tetracycline resistance (Tcr) gene of this vector. The pBR322 derivatives pBR327 and pBR328 lack this DNA sequence and are not mobilized by conjugation. Two derivatives of pBR328 were constructed by re-inserting the HaeIII-2 fragment in both orientations into the chloramphenicol-resistance gene of the same vector. One orientation of the HaeIII-2 fragment permitted mobilization by conjugation while the opposite orientation prevented mobilization. Further examination of pBR322 and derivatives revealed that the region between the origin of replication and Tcr gene also plays a role in regulating plasmid copy number.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacteriocin Plasmids; Cloning, Molecular/methods; DNA Replication; DNA, Bacterial/genetics; DNA, Recombinant; Escherichia coli/genetics; Genes; Genetic Vectors; Plasmids
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.