PMID:6255065
| Citation |
Hayzer, DJ and Leisinger, T (1980) The gene-enzyme relationships of proline biosynthesis in Escherichia coli. J. Gen. Microbiol. 118:287-93 |
|---|---|
| Abstract |
A simple chromatographic procedure has been devised to separate gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase, allowing the measurement of the former in crude Escherichia coli extracts. Analysis of a number of strains of E. coli has demonstrated that gene proA codes for gamma-glutamyl phosphate reductase and proB for gamma-glutamyl kinase. Introduction of a ColE1 hybrid plasmid containing the proA,B region into a strain with a chromosomal deletion of proA,B led to 3- and 17-fold increases in the specific activities of gamma-glutamyl kinase and gamma-glutamyl phosphate reductase, respectively. |
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| Keywords |
Aldehyde Oxidoreductases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Genotype; Glutamate-5-Semialdehyde Dehydrogenase; Mutation; Phosphotransferases/metabolism; Phosphotransferases (Carboxyl Group Acceptor); Plasmids; Proline/biosynthesis; Pyrroline Carboxylate Reductases/metabolism |
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