PMID:6247616
| Citation |
Danchin, A and Dondon, L (1980) Serine sensitivity of Escherichia coli K 12: partial characterization of a serine resistnat mutant that is extremely sensitive to 2-ketobutyrate. Mol. Gen. Genet. 178:155-64 |
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| Abstract |
E. coli wild type bacteria display sensitivity towards serine. A selection medium is described which allows selection of serine resistant mutants. One such mutant is described which presents pleiotropic alterations: it exhibits a thermosensitive growth pattern, alteration in the metabolism of the pppGpp and ppGpp nucleotides, cAMP intracellular level alteration, extreme sensitivity to 2-ketobutyric acid and a defect in the phosphotransferases permeation system. A conjecture explaining these apparently unrelated defects supposes that serine metabolism interferes via phosphoenol pyruvate with a cytoplasmic control of membrane activity (the mutant would be defective in the coupling between membrane and the protein responsible for its cytoplasmic control) and that 2-ketobutyrate is an effector of this activity. |
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| Keywords |
Butyrates/pharmacology; Culture Media; Cyclic AMP/metabolism; Drug Resistance, Microbial; Escherichia coli/genetics; Escherichia coli/metabolism; Guanosine Pentaphosphate/metabolism; Guanosine Tetraphosphate/metabolism; Keto Acids/pharmacology; Mutation; Phenotype; Serine/pharmacology |
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