PMID:6238175
| Citation |
Bear, SE, Court, DL and Friedman, DI (1984) An accessory role for Escherichia coli integration host factor: characterization of a lambda mutant dependent upon integration host factor for DNA packaging. J. Virol. 52:966-72 |
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| Abstract |
Bacteriophage lambda grows lytically on Escherichia coli defective for integration host factor, a protein involved in lambda site-specific recombination and the regulation of gene expression. We report the characterization of a mutant, lambda cos154, that, unlike wild-type lambda, is defective for growth in integration host factor-defective E. coli. The cis-dominant mutation in lambda cos154 is a single base pair change in a region of hyphenated dyad symmetry close to the lambda left cohesive end; this mutation prevents DNA packaging. We propose the following two alternative roles for this site in lambda DNA packaging: (i) to bind an E. coli accessory protein required in the absence of integration host factor or (ii) to bind the phage-encoded terminase protein that is essential for DNA packaging. |
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| Keywords |
Bacterial Proteins/genetics; Bacteriophage lambda/genetics; Bacteriophage lambda/growth & development; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Integrases; Mutation; Viral Proteins/genetics; Virus Replication |
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