PMID:6237244
| Citation |
Murakami, H, Kita, K, Oya, H and Anraku, Y (1984) Chromosomal location of the Escherichia coli cytochrome b556 gene, cybA. Mol. Gen. Genet. 196:1-5 |
|---|---|
| Abstract |
The amounts of cytochrome b556 in the cytoplasmic membranes of several Escherichia coli K12 strains having F-prime factors and a lambda transducing phage were determined. The amount was amplified about two-fold in strains having F100-12 and F152, but not in strains having F100-11, F8 and lambda psu+2glnS+. The strain TK3D11, which lacks the kdp-gltA region (deletion D-01) of the E. coli chromosome, did not synthesize cytochrome b556 at all. From these results, the gene cybA encoding cytochrome b556 was located in the kdp-gltA region. In the cytochrome b556-deficient mutant, a novel b type cytochrome, cytochrome b561 which is a product of the gene cybB, was identified. It seems to function as a physiological electron transferring cytochrome in place of cytochrome b556 in this mutant. |
| Links | |
| Keywords |
Bacteriophage lambda/genetics; Chromosome Mapping; Chromosomes, Bacterial/ultrastructure; Cytochrome b Group/genetics; Escherichia coli/genetics; F Factor; Gene Amplification; Genes, Bacterial |
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