PMID:6229418
| Citation |
Duncan, K, Lewendon, A and Coggins, JR (1984) The purification of 5-enolpyruvylshikimate 3-phosphate synthase from an overproducing strain of Escherichia coli. FEBS Lett. 165:121-7 |
|---|---|
| Abstract |
The Escherichia coli aroA gene which codes for the enzyme 5-enolpyruvylshikimate 3-phosphate synthase (EPSP synthase) has been cloned from the lambda-transducing bacteriophage lambda pserC. The gene has been located on a 4.7 kilobase pair PstI DNA fragment which has been inserted into the multiple copy plasmid pAT153. E. coli cells transformed with this recombinant plasmid overproduce EPSP synthase 100-fold. A simple method for the purification of homogeneous enzyme in milligram quantities has been devised. The resulting enzyme is indistinguishable from enzyme isolated from untransformed E. coli. |
| Links | |
| Keywords |
3-Phosphoshikimate 1-Carboxyvinyltransferase; Alkyl and Aryl Transferases; Bacteriophage lambda/genetics; Chromatography, Gel; DNA, Recombinant/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Kinetics; Molecular Weight; Peptide Fragments; Transferases/isolation & purification |
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