PMID:6184364

Citation	Blinkowa, A, Haldenwang, WG, Ramsey, JA, Henson, JM, Mullin, DA and Walker, JR (1983) Physiological properties of cold-sensitive suppressor mutations of a temperature-sensitive dnaZ mutant of Escherichia coli. J. Bacteriol. 153:66-75
Abstract	Suppressors of a temperature-sensitive dnaZ polymerization mutant of Escherichia coli have been identified by selecting temperature-insensitive revertants. Those suppressed strains which concomitantly became cold sensitive were chosen for further study. Intragenic suppressor mutations, which caused cold-sensitive defects in DNA polymerization, were located in dnaZ by transduction with lambda dnaZ+ phages. Extragenic suppressor mutations were mapped within the initiation gene dnaA. These suppressor-containing strains were defective in initiation at low temperature as determined by measurements of DNA synthesis in vivo and in toluene-treated cells. The occurrence of suppressor mutations of dnaZ(Ts) within the dnaA gene is considered evidence that the dnaA and dnaZ products interact in vivo. A second indication of a dnaA-dnaZ protein-protein interaction was provided by the observation that the introduction of additional copies of the dnaZ+ gene into a strain carrying the dnaA suppressor mutation was lethal [whether the strain was dnaZ+ or dnaZ(Ts)].
Links	PubMed PMC217342
Keywords	Bacterial Proteins/genetics; Cold Temperature; DNA Replication; DNA, Bacterial/biosynthesis; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Mutation; Plasmids; RNA, Bacterial/biosynthesis; Suppression, Genetic
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