PMID:6160371

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Citation

Cass, LG, Horwitz, AH, Miyada, CG, Greenfield, L and Wilcox, G (1980) The araC regulatory gene mRNA contains a leader sequence. Mol. Gen. Genet. 180:219-26

Abstract

An estimation of the size of the araC gene in Escherichila coli B/r was made by sub-cloning restriction fragments of the araC-containing hybrid plasmid pTB1 into the plasmid pBR322. Plasmids which contained a functional araC gene were identified by genetic complementation tests. DNA sequence analysis of the promoter-proximal region of the araC gene revealed that araC mRNA contains a 150 nucleotide leader.

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Keywords

Arabinose/genetics; Base Sequence; DNA, Bacterial/analysis; Escherichia coli/genetics; Genes, Regulator; Genetic Complementation Test; Nucleic Acid Hybridization; RNA, Bacterial/analysis; RNA, Messenger/analysis; RNA, Messenger/genetics

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