PMID:6139280
| Citation |
Lundberg, LG, Thoresson, HO, Karlström, OH and Nyman, PO (1983) Nucleotide sequence of the structural gene for dUTPase of Escherichia coli K-12. EMBO J. 2:967-71 |
|---|---|
| Abstract |
The nucleotide sequence of the dUTPase structural gene, dut, of Escherichia coli has been determined. The DNA sequence predicts a polypeptide chain of 150 amino acid residues (mol. wt. 16 006) corresponding in size and composition to the purified dUTPase subunit. In a tentative promoter region preceding the dut gene, the -35 and -10 regions are separated by a SacI (SstI) site. Cloning of the dut gene utilization this SacI site was previously shown to reduce dut expression dramatically. The nucleotide sequence also contains a 210-codon open reading frame 106 bp downstream of dut and co-directional with dut. Previous protein synthesis experiments using dut plasmids allocated the gene of a polypeptide of mol. wt. 23 500 to this DNA region. The open reading frame thus may correspond to a protein of unknown function co-transcribed with the dut gene. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Codon; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Pyrophosphatases/genetics |
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