PMID:6120930
| Citation |
Chen, YM, Backman, K and Magasanik, B (1982) Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli. J. Bacteriol. 150:214-20 |
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| Abstract |
DNA was prepared from a strain of Escherichia coli bearing a mutation which confers the GlnC phenotype (inability to reduce the expression of glnA and other nitrogen-regulated operons in response to ammonia in the growth medium). A fragment of this DNA carrying glnA, the structural gene for glutamine synthetase, was cloned on plasmid pBR322. By using recombination in vitro, we mapped the GlnC mutation to a region between glnA and glnG. This region defines a gene, glnL, which codes for a trans-acting product; the GlnC mutant produces an altered product. The glnL product plays a key role in the communication of information concerning the quality and abundance of the nitrogen source in the growth medium to a destination responsible for the regulation of glnA and other genes for enzymes responsible for nitrogen utilization. |
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| Keywords |
Ammonia/pharmacology; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Genes, Regulator; Glutamate-Ammonia Ligase/genetics; Histidine Ammonia-Lyase/metabolism; Mutation; Nitrogen/metabolism; Operon |
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