PMID:6117826
| Citation |
Nakamura, M, Yamada, M, Hirota, Y, Sugimoto, K, Oka, A and Takanami, M (1981) Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12. Nucleic Acids Res. 9:4669-76 |
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| Abstract |
We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin, ori, and asnA. The complete nucleotide sequence of the asnA gene was determined: the region of the structural gene extends 990 base-pairs at nucleotide positions 1434-2423 (see Fig. 3), which codes for a polypeptide of 330 amino-acid residues with a molecular weight of 36,688 daltons. The nucleotide sequences of the promoter and the ribosome-binding site of the gene are also assigned. We discuss the properties of its polypeptide. |
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| Keywords |
Amino Acid Sequence; Aspartate-Ammonia Ligase/genetics; Base Composition; Base Sequence; Cloning, Molecular; Codon; DNA Replication; DNA Restriction Enzymes; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Ligases/genetics |
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