PMID:6094478
| Citation |
Barbeyron, T, Kean, K and Forterre, P (1984) DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage. J. Bacteriol. 160:586-90 |
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| Abstract |
We have examined the presence of methylated adenine at GATC sequences (Dam phenotype) in the DNA of 23 eubacteria and 13 archaebacteria by using isoshizomer restriction enzymes. We have found a completely Dam+ phenotype in bacteria of nine genera related to the families Enterobacteriaceae, Parvobacteriaceae, and Vibrionaceae, and in the five cyanobacteria tested. We have found a partial Dam+ phenotype in the two archaebacteria Halobacterium saccharovorum and Methanobacterium sp. strain Ivanov. All of the other archaebacteria (three genera) and eubacteria (nine genera) tested were Dam-. Phylogenetic analysis, based on the evolutionary tree of Fox et al. (Science 209:457-463, 1980), indicates that dam methylation in the Escherichia coli lineage appeared recently in bacterial evolution and is restricted to a small range of closely related bacteria. |
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| Keywords |
Adenine/analogs & derivatives; Archaea/genetics; Bacteria/genetics; Base Sequence; DNA Restriction Enzymes; DNA, Bacterial/genetics; Escherichia coli/genetics; Gram-Negative Bacteria/genetics; Methylation; Phylogeny; Species Specificity |
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