PMID:6091903

Citation	Fuller, RS, Funnell, BE and Kornberg, A (1984) The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites. Cell 38:889-900
Abstract	Specific binding of dnaA protein to the E. coli origin of replication (oriC) is essential for initiation of chromosomal replication. Based on binding of plasmid DNA fragments, dnaA protein recognizes in addition to oriC a number of specific sites: within or near the replication origins of pSC101, pBR322, and ColE1; within the regulatory regions of the dnaA and "X-protein" genes; and in IRL-Tn5. All strong binding sites share a common 9 bp sequence, 5'-TTATCACACAA, repeated at four conserved positions within oriC. As shown by DNAase I footprinting, dnaA protein binding is highly cooperative, covering 250 bp at oriC and 100 bp at single 9 bp sequence sites. Consistent with filter-binding and nuclease-protection studies, complexes of 20 to 30 dnaA monomers are visualized at oriC and other sites by electron microscopy. The functions of dnaA complexes and 9 bp sequences at these sites are discussed.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Chromosomes, Bacterial/physiology; DNA Replication; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Escherichia coli/genetics; Genes, Regulator; Kinetics; Microscopy, Electron; Protein Binding
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