PMID:6089341
| Citation |
Van Dyke, MM and Dervan, PB (1984) Echinomycin binding sites on DNA. Science 225:1122-7 |
|---|---|
| Abstract |
The preferred binding sites of echinomycin on DNA can be determined by a method called "footprinting." A 32P end-labeled restriction fragment from pBR322 DNA is protected by binding to echinomycin, and cleaved by a synthetic DNA cleaving reagent, methidiumpropyl--EDTA . Fe(II); the DNA cleavage products are then subjected to high-resolution gel analyses. This method reveals that echinomycin has a binding site size of four base pairs. The strong binding sites for echinomycin contain the central two-base-pair sequence 5'-CG-3'. From an analysis of 15 echinomycin sites on 210 base pairs of DNA, key recognition elements for echinomycin are contained in the sequences (5'-3') ACGT and TCGT (A, adenine; C, cytosine; G, guanine; T, thymine). |
| Links | |
| Keywords |
Base Sequence; Binding Sites; DNA/metabolism; DNA Restriction Enzymes; Echinomycin/metabolism; Electrophoresis; Escherichia coli/genetics; Plasmids; Quinoxalines/metabolism |
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