PMID:4945185
| Citation |
Eisenberg, MA and Stoner, GL (1971) Biosynthesis of 7,8-diaminopelargonic acid, a biotin intermediate, from 7-keto-8-aminopelargonic acid and S-adenosyl-L-methionine. J. Bacteriol. 108:1135-40 |
|---|---|
| Abstract |
Resting cells of Escherichia coli strain D302(bioD302) can synthesize 7,8-diaminopelargonic acid from 7-keto-8-aminopelargonic acid. The product of this aminotransferase reaction has been identified by paper chromatography and electrophoresis. Glucose enhances the vitamer yield twofold. Of the 19 amino acids tested as amino donors, only methionine proved to be significantly stimulatory. In cell-free extracts, however, methionine was completely inactive unless both adenosine triphosphate (ATP) and Mg(2+) were present. S-Adenosyl-l-methionine (SAM) was about 10 times more effective than methionine, ATP, and Mg(2+). The optimal conditions for the reaction were determined, and substrate inhibition was found for 7-keto-8-aminopelargonic acid. It has been possible to eliminate certain impurities as amino donors in the commercial preparation of SAM and those that may arise in enzymatic reactions in which SAM is a substrate. The direct participation of SAM in the aminotransferase reaction seems a likely possibility. |
| Links | |
| Keywords |
Adenosine/metabolism; Adenosine Triphosphate/metabolism; Amines/biosynthesis; Amines/metabolism; Amino Acids/metabolism; Biotin/biosynthesis; Cell-Free System; Chromatography, Paper; Culture Media; Electron Transport; Electrophoresis, Paper; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Fatty Acids/biosynthesis; Fatty Acids/metabolism; Glucose/metabolism; Keto Acids/metabolism; Ketones/metabolism; Magnesium/metabolism; Methionine/metabolism; S-Adenosylmethionine/metabolism; Transaminases/metabolism |
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