PMID:4943555
| Citation |
Lennette, ET, Gorelic, L and Apirion, D (1971) An Escherichia coli mutant with increased messenger ribonuclease activity. Proc. Natl. Acad. Sci. U.S.A. 68:3140-4 |
|---|---|
| Abstract |
A temperature-sensitive mutant strain of Escherichia coli exhibits a remarkable increase in RNase activity when grown at its nonpermissive temperature. During growth at the nonpermissive temperature, there is an increase in the extent of breakdown of pulse-labeled RNA and a decrease in the functional lifetime of the mRNA for the lac operon. T7 RNA, which is usually stable in E. coli, is also stable in this strain at the nonpermissive temperature. It is possible that the RNase measured is part of the normal mechanism of mRNA degradation in the cell. A mechanism for mRNA degradation that requires the combined action of endonuclease(s) and 3' to 5' exonuclease(s) is proposed. |
| Links | |
| Keywords |
Biodegradation, Environmental; Coliphages; Culture Media; Deoxyribonucleases; Escherichia coli/enzymology; Escherichia coli/metabolism; Galactosidases/metabolism; Lactose/biosynthesis; Mutation; Operon; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Ribonucleases/metabolism; Temperature; Time Factors; Tritium; Uridine |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.