PMID:4934524
| Citation |
Cassuto, E, Lash, T, Sriprakash, KS and Radding, CM (1971) Role of exonuclease and beta protein of phage lambda in genetic recombination. V. Recombination of lambda DNA in vitro. Proc. Natl. Acad. Sci. U.S.A. 68:1639-43 |
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| Abstract |
The sequential action of lambda exonuclease and polynucleotide ligase upon redundant joint molecules is sufficient to produce intact polynucleotide chains and heat-stable, biologically active molecules of lambda DNA, whereas the action of ligase alone is insufficient. These results (a) confirm the previously described mechanism of single-strand assimilation, including a subsidiary mechanism by which the further action of lambda exonuclease is arrested when a redundant strand is completely assimilated, and (b) represent a simulation of the steps in genetic recombination that follow the formation of biparental complexes (synapsis). lambda exonuclease is postulated to catalyze a concerted reaction that includes exposure of complementary sequences, formation of heteroduplex regions, and elimination of redundant branches. |
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| Keywords |
Biological Assay; Chromatography, DEAE-Cellulose; Coliphages/enzymology; DNA, Viral; Deoxyribonucleases; Escherichia coli; Genetics, Microbial; Ligases; Phosphorus Isotopes; Polynucleotides; RNA, Transfer; Recombination, Genetic; Tritium |
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