PMID:4927675
| Citation |
Kushner, SR, Nagaishi, H, Templin, A and Clark, AJ (1971) Genetic recombination in Escherichia coli: the role of exonuclease I. Proc. Natl. Acad. Sci. U.S.A. 68:824-7 |
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| Abstract |
The indirect suppression of recB(-) and recB(-)recC(-) mutations by the sbcB(-) allele is caused by the loss of a nuclease active on denatured DNA. Results from enzyme purifications and studies with a specific antiserum demonstrate that the activity present in sbcB(+) strains, and lost in sbcB(-) strains, is exonuclease I. It is likely that sbcB is the structural gene for exonuclease I. The loss of exonuclease I activity restores the recombination proficiency of Escherichia coli cells that has been lost by mutations in the recB and/or recC genes. This indicates that in the absence of the recB-recC-determined enzyme, exonuclease I prevents recombination. Hypothetical pathways illustrating this conclusion are presented. |
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| Keywords |
Alleles; DNA; Deoxyribonucleases/isolation & purification; Deoxyribonucleases/physiology; Escherichia coli/enzymology; Genes; Immune Sera/pharmacology; Mutation; Recombination, Genetic; Transduction, Genetic |
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