PMID:4914079
| Citation |
Zwaig, N, Kistler, WS and Lin, EC (1970) Glycerol kinase, the pacemaker for the dissimilation of glycerol in Escherichia coli. J. Bacteriol. 102:753-9 |
|---|---|
| Abstract |
The activity of glycerol kinase is rate-limiting in the metabolism of glycerol by cells of Escherichia coli. A mutant strain producing a glycerol kinase resistant to inhibition by fructose-1,6-diphosphate grows faster than its wild-type parent on glycerol as the sole source of carbon and energy. The amount of intracellular fructose-1,6-diphosphate was determined for wild-type cells growing exponentially on glycerol. The water content of such cells was also determined, allowing calculation of the intracellular concentration of fructose-1,6-diphosphate. This value, 1.7 mm, is adequate to exert substantial inhibition on the wild-type glycerol kinase. The desensitization of glycerol kinase to feedback inhibition also enhances the power of glycerol to exert catabolite repression, both on the enzymes of the glycerol system itself and on those of the lactose system. However, desensitization of glycerol kinase alone does not eliminate the phenomenon of diauxic growth in a glucose-glycerol medium. Biphasic growth in such a medium is abolished if the altered enzyme is produced constitutively. The constitutive production of the mutant kinase at high levels, however, renders the cells vulnerable to glycerol. Thus, when the cells have been grown on a carbon source with a low power for catabolite repression, e.g., succinate, sudden exposure to glycerol leads to overconsumption of the nutrient and cell death. |
| Links | |
| Keywords |
Carbon Isotopes; Colorimetry; Culture Media; Escherichia coli/analysis; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Filtration; Galactosidases/metabolism; Glucose/metabolism; Glucosephosphate Dehydrogenase/metabolism; Glycerol/metabolism; Hexosephosphates/analysis; Hexosephosphates/pharmacology; Inulin/metabolism; Mutation; Phosphotransferases/antagonists & inhibitors; Phosphotransferases/metabolism; Succinates/metabolism; Transduction, Genetic; Water/analysis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.