PMID:4908790
| Citation |
Lieberman, MM and Markovitz, A (1970) Depression of guanosine diphosphate-mannose pyrophosphorylase by mutations in two different regulator genes involved in capsular polysaccharide synthesis in Escherichia coli K-12. J. Bacteriol. 101:965-72 |
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| Abstract |
Mutations in a regulator gene (capR) that causes increased synthesis of capsular polysaccharide and derepressed synthesis of several enzymes involved in polysaccharide synthesis also derepress synthesis of guanosine diphosphate (GDP)-mannose pyrophosphorylase. In addition, a second mucoid mutation (capS, which maps separately from capR) also results in the derepression of GDP-mannose pyrophosphorylase. New conditions for assaying GDP-mannose hydrolyase and GDP-l-fucose synthetase permitted us to show that these enzymes are also derepressed in the capS mucoid strain. Although phosphomannose isomerase and uridine diphosphate-galactose-4-epimerase are derepressed in capR mucoid strains, they are not derepressed in capS mucoid strains. A nonmucoid mutant of a strain containing the capR9 (mucoid) allele was deficient in GDP-mannose pyrophosphorylase. |
| Links | |
| Keywords |
Cell Wall/metabolism; Escherichia coli/enzymology; Escherichia coli/metabolism; Genes, Regulator; Genetics, Microbial; Glycoside Hydrolases/metabolism; Guanine Nucleotides; Isomerases/metabolism; Ligases/metabolism; Mutation; Nucleotidyltransferases/metabolism; Polysaccharides, Bacterial/biosynthesis; Uracil Nucleotides |
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