PMID:4862193
Citation |
Markovitz, A, Sydiskis, RJ and Lieberman, MM (1967) Genetic and biochemical studies on mannose-negative mutants that are deficient in phosphomannose isomerase in Escherichia coli K-12. J. Bacteriol. 94:1492-6 |
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Abstract |
Two mannose-negative mutants of Escherichia coli K-12 have been isolated. These mutants are deficient in the ability to synthesize phosphomannose isomerase and capsular polysaccharide when grown on glucose-containing media. Interrupted mating experiments to determine the kinetics of genetic transfer show that the two mannose-negative mutations map together between the histidine and tryptophan regions of the E. coli chromosome. |
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Keywords |
Chromosome Mapping; Chromosomes, Bacterial; Conjugation, Genetic; Escherichia coli/enzymology; Escherichia coli/growth & development; Genetics, Microbial; Glucose/metabolism; Glucose-6-Phosphate Isomerase/metabolism; Glucosephosphate Dehydrogenase/metabolism; Histidine; Isomerases/biosynthesis; Isomerases/metabolism; Mannose/metabolism; Mutation; Polysaccharides, Bacterial/biosynthesis; Recombination, Genetic; Streptomycin/pharmacology; Tryptophan |
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