PMID:4604283
| Citation |
Neidhardt, FC, Bloch, PL and Smith, DF (1974) Culture medium for enterobacteria. J. Bacteriol. 119:736-47 |
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| Abstract |
A new minimal medium for enterobacteria has been developed. It supports growth of Escherichia coli and Salmonella typhimurium at rates comparable to those of any of the traditional media that have high phosphate concentrations, but each of the macronutrients (phosphate, sulfate, and nitrogen) is present at a sufficiently low level to permit isotopic labeling. Buffering capacity is provided by an organic dipolar ion, morpholinopropane sulfonate, which has a desirable pK (7.2) and no apparent inhibitory effect on growth. The medium has been developed with the objectives of (i) providing reproducibility of chemical composition, (ii) meeting the experimentally determined nutritional needs of the cell, (iii) avoiding an unnecessary excess of the major ionic species, (iv) facilitating the adjustment of the levels of individual ionic species, both for isotopic labeling and for nutritional studies, (v) supplying a complete array of micronutrients, (vi) setting a particular ion as the crop-limiting factor when the carbon and energy source is in excess, and (vii) providing maximal convenience in the manufacture and storage of the medium. |
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| Keywords |
Ammonia; Buffers; Calcium; Cell Count; Culture Media; Enterobacteriaceae/growth & development; Escherichia coli/growth & development; Glucose; Hydrogen-Ion Concentration; Iron; Magnesium; Phosphates; Salmonella typhimurium/growth & development; Sodium Chloride; Sulfates |
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This paper describes the development of MOPS minimal medium, which is a preferred medium for rigorous physiological studies of E. coli. MOPS medium is especially useful for studies where phosphate is limiting; other defined media such as M9 use phosphate as the buffer.
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