PMID:4591470
| Citation |
Bergquist, PL and Adelberg, EA (1972) Abnormal excision and transfer of chromosomal segments by a strain of Escherichia coli K-12. J. Bacteriol. 111:119-28 |
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| Abstract |
PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the excision and transfer of extended chromosomal segments which include the integrated F-gal; the excised segments are inferred to have circularized. Their excision, which is independent of the recA(+) allele, occurs at an unusually high rate during conjugation; a mutant F-initiator protein is suggested as the cause of this phenomenon. After their establishment in recipients, the enlarged F-genotes undergo further deletions of included donor genes by a process which is again recA(+)-independent. In Rec(+), but not in Rec(-), cells, a high proportion of the deleted fragments are rescued by integration into the recipient's chromosome. |
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| Keywords |
Acridines/pharmacology; Chromosome Mapping; Chromosomes, Bacterial; Conjugation, Genetic; Crosses, Genetic; DNA Replication; Escherichia coli/metabolism; Galactose/metabolism; Genes; Genetic Linkage; Leucine/biosynthesis; Mutation; Proline/biosynthesis; Recombination, Genetic; Temperature; Threonine/biosynthesis; Xylose/metabolism |
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