PMID:4583243
| Citation |
Celis, TF, Rosenfeld, HJ and Maas, WK (1973) Mutant of Escherichia coli K-12 defective in the transport of basic amino acids. J. Bacteriol. 116:619-26 |
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| Abstract |
Escherichia coli K-12 possesses two active transport systems for arginine, two for ornithine, and two for lysine. In each case there is a low- and a high-affinity transport system. They have been characterized kinetically and by response to competitive inhibition by arginine, lysine, ornithine and other structurally related amino acids. Competitors inhibit the high-affinity systems of the three amino acids, whereas the low-affinity systems are not inhibited. On the basis of kinetic evidence and competition studies, it is concluded that there is a common high-affinity transport system for arginine, ornithine, and lysine, and three low-affinity specific ones. Repression studies have shown that arginine and ornithine repress each other's specific transport systems in addition to the repression of their own specific systems, whereas lysine represses its own specific transport system. The common transport system was found to be repressible only by lysine. A mutant was studied in which the uptake of arginine, ornithine, and lysine is reduced. The mutation was found to affect both the common and the specific transport systems. |
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| Keywords |
Arginine/metabolism; Biological Transport; Carbon Radioisotopes; Chromosome Mapping; Chromosomes, Bacterial; Culture Media; Enzyme Repression; Escherichia coli/metabolism; Genetic Linkage; Histidine/metabolism; Lysine/metabolism; Mutation; Ornithine/metabolism; Putrescine/biosynthesis; Time Factors; Tritium |
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