PMID:4572721
| Citation |
Young, IG, Stroobant, P, Macdonald, CG and Gibson, F (1973) Pathway for ubiquinone biosynthesis in Escherichia coli K-12: gene-enzyme relationships and intermediates. J. Bacteriol. 114:42-52 |
|---|---|
| Abstract |
Seven ubiquinone-deficient mutants of Escherichia coli, each of which accumulates two phenolic precursors of ubiquinone, have been characterized, and the accumulated compounds have been identified. The mutants accumulate small quantities of 2-octaprenyl-6-methoxyphenol, which was isolated and characterized by nuclear magnetic resonance and mass spectrometry, and relatively large amounts of 2-octaprenylphenol, a compound previously identified from E. coli. They also accumulate small quantities of a compound identified as 2-(hydroxyoctaprenyl)phenol although the relevance of this compound to the biosynthesis of ubiquinone is not clear. The results of genetic analysis suggest that each of the mutants carries a mutation in a gene (designated ubiH) which is located at about min 56 on the E. coli chromosome and is co-transducible with the serA and lysB genes. Based on information obtained from this and previous studies with ubiquinone-deficient mutants, a pathway is proposed for ubiquinone biosynthesis in E. coli, and a summary of the known gene-enzyme relationships is given. |
| Links | |
| Keywords |
Chromatography, Thin Layer; Chromosome Mapping; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes; Magnetic Resonance Spectroscopy; Mass Spectrometry; Mutation; Phenols/analysis; Phenols/isolation & purification; Ubiquinone/analysis; Ubiquinone/biosynthesis; Ubiquinone/isolation & purification; Vinyl Compounds/isolation & purification |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.