PMID:4570768
| Citation |
Clarke, SJ, Low, B and Konigsberg, WH (1973) Close linkage of the genes serC (for phosphohydroxy pyruvate transaminase) and serS (for seryl-transfer ribonucleic acid synthetase) in Escherichia coli K-12. J. Bacteriol. 113:1091-5 |
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| Abstract |
Escherichia coli strain K28, isolated after nitrosoguanidine mutagenesis, was found to be auxotrophic for serine. It was also temperature sensitive for growth as a result of producing an altered seryl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.11, l-serine: tRNA ligase [AMP]). The auxotrophy was caused by a mutation in the structural gene for phosphohydroxy-pyruvate transaminase (serC), which was distinct from, but closely linked to, the structural gene for seryl-tRNA synthetase (serS). We conclude that the relevant genes are in the order gal-serS-serC-aroA. |
| Links | |
| Keywords |
Amino Acyl-tRNA Synthetases/biosynthesis; Amino Acyl-tRNA Synthetases/metabolism; Carbon Isotopes; Cell-Free System; Chromosome Mapping; Crosses, Genetic; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes; Genetic Linkage; Mutagens; Mutation; Nitrosoguanidines; Pyruvates; Recombination, Genetic; Serine/metabolism; Temperature; Transaminases/biosynthesis; Transaminases/metabolism; Transduction, Genetic |
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