PMID:4567136
| Citation |
Cox, R and Charles, HP (1973) Porphyrin-accumulating mutants of Escherichia coli. J. Bacteriol. 113:122-32 |
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| Abstract |
Four mutants (pop-1, pop-6, pop-10, and pop-14) which accumulate a red water-insoluble pigment were obtained in Escherichia coli K-12 AB1621. For each mutant, the red pigment was shown to be protoporphyrin IX, a late precursor of heme. Mutagenic treatment of mutant pop-1 yielded a secondary mutant, pop-1 sec-20, which accumulated a brown water-soluble pigment. The brown pigment was shown to be coproporphyrin III. Mutant pop-1 resembled the parental strain in its cytochrome absorption spectrum, catalase activity, and ability to grow on nonfermentable carbon and energy sources; therefore, its ability to produce and utilize heme was unimpaired. Judged on the same criteria, the secondary mutant, pop-1 sec-20, was partially heme and respiratory deficient. Growth in anaerobic conditions decreased by 25% the accumulation of protoporphyrin by pop-1; under the same conditions, pop-1 sec-20 did not accumulate coproporphyrin or coproporphyrinogen. The mutations causing protoporphyrin accumulation in all four pop mutants were found to map in the lac to purE (10-13 min) region of the E. coli chromosome. In the case of mutant pop-1, the mutation was shown to be strongly linked to the tsx locus (12 min). In mutant pop-1 sec-20, the second mutation causing coproporphyrin accumulation was co-transducible with the gal locus at a frequency of 88 to 96%. The mechanism of porphyrin accumulation by the mutants is discussed. |
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| Keywords |
Aerobiosis; Anaerobiosis; Catalase/metabolism; Chromatography, Paper; Chromosome Mapping; Conjugation, Genetic; Culture Media; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Heme/biosynthesis; Mutation; Porphyrins/analysis; Porphyrins/isolation & purification; Porphyrins/metabolism; Recombination, Genetic; Spectrum Analysis; Transduction, Genetic |
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