PMID:4565531
| Citation |
Langman, L, Young, IG, Frost, GE, Rosenberg, H and Gibson, F (1972) Enterochelin system of iron transport in Escherichia coli: mutations affecting ferric-enterochelin esterase. J. Bacteriol. 112:1142-9 |
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| Abstract |
Three mutant strains of Escherichia coli have been isolated which are lacking ferric-enterochelin esterase activity. This enzyme catalyzes the hydrolysis of the enterochelin moiety of ferric-enterochelin to yield ultimately three molecules of N-2,3-dihydroxybenzoylserine. The mutants (designated fes(-)) were shown to be unaffected in enterochelin biosynthesis, capable of enterochelin-mediated iron uptake, and able to utilize ferric-dihydroxybenzoylserine complexes normally. When grown under iron-deficient conditions, however, they showed an absolute requirement for added iron or citrate, a phenotype characteristic of mutants defective in some part of the enterochelin system of iron uptake. These results support the theory that iron, taken up by the cell as ferric-enterochelin is only available for general cell metabolism after hydrolysis of the ligand by enterochelin esterase. The three fes(-) strains were shown to be affected in the B component of enterochelin esterase. The fesB gene which is probably the structural gene coding for component B of the esterase, was shown to be located at about minute 14 on the E. coli chromosome together with seven other genes involved in the enterochelin system of iron transport. |
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| Keywords |
Benzoates; Biological Transport; Catalysis; Catechols; Cell-Free System; Chromatography, Gel; Chromosome Mapping; Chromosomes, Bacterial; Culture Media; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Esterases/metabolism; Genes; Genetic Complementation Test; Hydrolysis; Iron/metabolism; Iron Chelating Agents/biosynthesis; Iron Chelating Agents/metabolism; Iron Isotopes; Mutation; Serine; Transduction, Genetic |
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