PMID:4564200
| Citation |
Harder, ME, Beacham, IR, Cronan, JE Jr, Beacham, K, Honegger, JL and Silbert, DF (1972) Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties. Proc. Natl. Acad. Sci. U.S.A. 69:3105-9 |
|---|---|
| Abstract |
A procedure is described for selection of temperature-sensitive mutants affecting fatty-acid synthesis based upon radiation suicide of wild-type organisms by tritiated acetate selectively incorporated into fatty acids. At 37 degrees , two of the mutants extensively incorporate fatty-acid supplements provided in the medium, and grow for extended periods only when a trans-unsaturated or a combination of saturated and cis-unsaturated fatty acids is available. In vivo fatty-acid synthesis, measured by [(14)C]acetate incorporation, is temperature-sensitive in these strains relative to protein synthesis and other non-lipid macromolecular syntheses using acetate. The biochemical nature of these mutations has not been identified. |
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| Keywords |
Acetates/metabolism; Carbon Isotopes; Escherichia coli/analysis; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Fatty Acids/analysis; Fatty Acids/biosynthesis; Fatty Acids/metabolism; Fatty Acids, Unsaturated/metabolism; Glutamates/metabolism; Isotope Labeling; Mutation; Phosphatidylethanolamines/analysis; Temperature; Tritium |
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