PMID:4563986
| Citation |
Miyakawa, T, Matsuzawa, H, Matsuhashi, M and Sugino, Y (1972) Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis. J. Bacteriol. 112:950-8 |
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| Abstract |
Temperature-sensitive mutants of Escherichia coli K-12 which cannot form cell wall peptidoglycan at 42 C were isolated. The thermosensitive steps were characterized biochemically, and the genes coding the enzymes of peptidoglycan synthesis were mapped. These genes were in two clusters: one group, located at about 1.5 min between leu and azi, was designated as mra (murein a); the second group, located at about 77.5 min close to argH and metB, was designated as mrb (murein b, with the order metB-argH-mrb). No simple relations were found between the gene location and the order or localization of enzymes involved in the sequence of reactions of cell wall peptidoglycan biosynthesis. |
| Links | |
| Keywords |
Alanine/metabolism; Carbon Isotopes; Carboxypeptidases/metabolism; Cell Wall/metabolism; Cell-Free System; Chromatography, Paper; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Glucosamine/metabolism; Ligases/metabolism; Mutation; Oxidoreductases/metabolism; Peptide Synthases/metabolism; Peptidoglycan/biosynthesis; Phosphoenolpyruvate; Stereoisomerism; Temperature; Transduction, Genetic; Transferases/metabolism; Uridine/metabolism |
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