PMID:4559737
| Citation |
Solomon, E and Lin, EC (1972) Mutations affecting the dissimilation of mannitol by Escherichia coli K-12. J. Bacteriol. 111:566-74 |
|---|---|
| Abstract |
Mutants of Escherichia coli K-12 defective in the mannitol-specific enzyme II complex of the phosphoenolpyruvate phosphotransferase system (PTS) or lacking mannitol-1-phosphate dehydrogenase have been isolated. These mutants fail only to grow on mannitol. Growth of the dehydrogenase-negative mutant on casein hydrolysate can be abruptly inhibited by exposure to mannitol. A mutant with constitutive expression of both of these enzymes has also been isolated. All three mutations are clustered in a region represented at min 71 of the Taylor map. In a mutant with less than 5% of the activity of enzyme I of the PTS, both the enzyme II complex and the dehydrogenase remain inducible by mannitol. In the mutant defective in the enzyme II complex, mannitol is able to induce the dehydrogenase. Thus, mannitol, rather than its phosphorylated product, seems to be the inducer. |
| Links | |
| Keywords |
Alcohol Oxidoreductases/biosynthesis; Carbon Isotopes; Chromosome Mapping; Chromosomes, Bacterial; Conjugation, Genetic; Culture Media; Enzyme Induction; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Mannitol/metabolism; Mutation; Phosphoenolpyruvate; Phosphotransferases/biosynthesis; Recombination, Genetic; Transduction, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
