PMID:4554534
| Citation |
Platt, T, Weber, K, Ganem, D and Miller, JH (1972) Translational restarts: AUG reinitiation of a lac repressor fragment. Proc. Natl. Acad. Sci. U.S.A. 69:897-901 |
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| Abstract |
An early, spontaneous amber mutation in the lac i-gene allows translational reinitiation, which results in a mutant lac repressor. Comparison of the amino-terminal sequence of this mutant repressor with the partial amino-acid sequence of the wild-type lac repressor shows that reinitiation occurs at the first internal AUG codon, and results in a mutant protein lacking 42 residues at the amino-terminal end. This protein binds the inducer isopropyl-beta-D-thiogalactoside with normal affinity, and is capable of maintaining a tetrameric structure; however, it does not repress in vivo. These data suggest that the amino-terminal portion of the wild-type lac repressor is necessary either for direct binding to the lac operator or for the correct conformation for binding to DNA. |
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| Keywords |
Adenine Nucleotides/pharmacology; Amino Acid Sequence; Antigen-Antibody Complex; Bacterial Proteins/biosynthesis; Binding Sites; Electrophoresis; Escherichia coli/drug effects; Escherichia coli/metabolism; Genetic Code; Guanine Nucleotides/pharmacology; Lactose/biosynthesis; Mutation; Nucleic Acid Conformation; Peptide Biosynthesis; Peptide Chain Initiation, Translational/drug effects; Peptide Chain Termination, Translational/drug effects; Polynucleotides/pharmacology; Protein Binding/drug effects; Suppression, Genetic; Uracil Nucleotides/pharmacology |
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