PMID:4371357

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Citation

Artman, M and Werthamer, S (1974) Use of streptomycin and cyclic adenosine 5'-monophosphate in the isolation of mutants deficient in CAP protein. J. Bacteriol. 120:542-4

Abstract

A method is described for selective isolation of mutants deficient in CAP protein necessary for the expression of catabolite-sensitive operons in Escherichia coli.

Links

PubMed PMC245798

Keywords

Arabinose/metabolism; Bacterial Proteins/biosynthesis; Cyclic AMP/pharmacology; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Galactosidases/biosynthesis; Glucose/metabolism; Glycerol/metabolism; Lactose/metabolism; Leucine/metabolism; Mutagens; Mutation; Nitrosoguanidines; Nucleic Acid Hybridization; Operon; RNA, Bacterial/biosynthesis; RNA, Messenger/biosynthesis; Rhamnose/metabolism; Streptomycin/pharmacology; Tritium; Tryptophanase/biosynthesis; Uridine/metabolism

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