PMID:4321330
| Citation |
Dempsey, WB and Ito, H (1970) Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants. J. Bacteriol. 104:658-67 |
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| Abstract |
At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The aminotransferase isolated from this mutant has been compared to that isolated from wild-type E. coli and found to have apparently normal affinity for pyridoxal 5'-phosphate, but reduced affinity for pyridoxamine 5'-phosphate. |
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| Keywords |
Culture Media; Densitometry; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Genetics, Microbial; Glycerolphosphate Dehydrogenase/metabolism; Mutation; Phenotype; Phosphoric Monoester Hydrolases/metabolism; Pyridoxal Phosphate/pharmacology; Pyridoxine/biosynthesis; Pyridoxine/metabolism; Serine/metabolism; Serine/pharmacology; Transaminases/metabolism |
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