PMID:3907632
| Citation |
Malcolm, BA and Kirsch, JF (1985) Site-directed mutagenesis of aspartate aminotransferase from E. coli. Biochem. Biophys. Res. Commun. 132:915-21 |
|---|---|
| Abstract |
The gene for aspartate aminotransferase from E. coli (aspC) was subcloned into M13 phage and sequenced using the Sanger dideoxy method with synthetic oligonucleotide primers. A mutant gene was constructed using site-directed mutagenesis techniques in which the codon for the lysine that forms the Schiffs base with pyridoxal phosphate was replaced with one coding for alanine. The mutant gene was expressed under control of the Tac promoter to overproduce a mutant protein lacking enzymatic activity. |
| Links | |
| Keywords |
Aspartate Aminotransferases/genetics; Base Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Mutation |
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