PMID:3882663
| Citation |
Rule, GS, Pratt, EA, Chin, CC, Wold, F and Ho, C (1985) Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli. J. Bacteriol. 161:1059-68 |
|---|---|
| Abstract |
Recombinant DNA plasmids containing the gene for the membrane-bound D-lactate dehydrogenase (D-LDH) of Escherichia coli linked to the promoter PL from lambda were constructed. After induction, the levels of D-LDH were elevated 300-fold over that of the wild type and amounted to 35% of the total cellular protein. The nucleotide sequence of the D-LDH gene was determined and shown to agree with the amino acid composition and the amino-terminal sequence of the purified enzyme. Removal of the amino-terminal formyl-Met from D-LDH was not inhibited in cells which contained these high levels of D-LDH. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cell Membrane/enzymology; Codon; Enzyme Induction; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; L-Lactate Dehydrogenase/biosynthesis; L-Lactate Dehydrogenase/genetics; Plasmids |
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