PMID:384209
| Citation |
Raychowdhury, R, Schlesinger, DH and Wilson, DB (1979) E. coli galactose-1-phosphate uridyl transferase: N-terminal and C-terminal sequences. Mol. Cell. Biochem. 23:167-75 |
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| Abstract |
A modified procedure for the purification of E. coli galactose-1-phosphate uridyl transferase (E.C. 2.7.6.12) was developed which reproducibly gives pure enzyme. The purified enzyme was shown to be a dimeric protein with a subunit molecular weight of 41,000 and its amino acid composition and content of free sulfhydryl groups were determined. The N-terminal and C-terminal amino acid sequences were found to be NH2-thr-gln-phe-asn-pro-val-asp and -ser(val leu)-ala-COOH respectively. This N-terminal sequence allowed the identification of the start of the transferase gene in the DNA sequence determined by GRINDLEY. Furthermore it appears to define a nine base intercistronic region between the epimerase and transferase genes. |
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| Keywords |
Amino Acid Sequence; Amino Acids/analysis; Escherichia coli/enzymology; Escherichia coli/genetics; Methods; Molecular Weight; Nucleotidyltransferases/isolation & purification; Operon; UTP-Hexose-1-Phosphate Uridylyltransferase/genetics; UTP-Hexose-1-Phosphate Uridylyltransferase/isolation & purification |
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