PMID:372492
| Citation |
Alaeddinoglu, NG and Charles, HP (1979) Transfer of a gene for sucrose utilization into Escherichia coli K12, and consequent failure of expression of genes for D-serine utilization. J. Gen. Microbiol. 110:47-59 |
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| Abstract |
As the first stage in investigating the genetic basis of natural variation in Escherichia coli, the gene(s) conferring the ability to use sucrose as a carbon and energy source (given the symbol sac+) was transferred from a wild strain to K12, which does not use sucrose. The sac+ region was transferred by two different methods. On both occasions it took a chromosomal location at minute 50.5 on the linkage map, between aroC and supN, in the region of the dsd genes, which confer the ability to use D-serine as a carbon and energy source. When the sac+ region was present in the K12 chromosome the bacteria were unable to use D-serine as a carbon and energy source. In F' sac+/dsd+ diploids, the dsd+ genes were similarly not expressed. Strain K12(sac+) bacteria were sensitive to inhibition by D-serine; they mutated to D-serine resistance with much greater frequency than did a dsd mutant of K12. Such bacteria also mutated frequently to use raffinose. Strain K12(sac+) bacteria did not utilize sucrose when they carried a mutation affecting the phosphotransferase system. |
| Links | |
| Keywords |
Chromosome Mapping; Chromosomes, Bacterial/ultrastructure; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Genes; Genetic Linkage; R Factors; Serine/metabolism; Sucrose/metabolism; Transduction, Genetic |
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