PMID:358190
| Citation |
Dahlberg, AE, Dahlberg, JE, Lund, E, Tokimatsu, H, Rabson, AB, Calvert, PC, Reynolds, F and Zahalak, M (1978) Processing of the 5' end of Escherichia coli 16S ribosomal RNA. Proc. Natl. Acad. Sci. U.S.A. 75:3598-602 |
|---|---|
| Abstract |
We have isolated and partially characterized an endonuclease involved in processing the 5' end of 16S rRNA of Escherichia coli. A mutant strain that is deficient in this enzyme accumulates a new precursor of 16S rRNA, named 16.3S rRNA. This rRNA has the 3' end of mature 16S rRNA but is about 60 nucleotides longer at the 5' end. In vitro, the enzyme preparation cleaves an RNA fragment of about 60 nucleotides from the 5' end of 16.3S rRNA in 30S ribosomal subunits, yielding the mature 5' end of 16S rRNA. In the mutant strain the 16.3S rRNA is associated with a full complement of 21 ribosomal proteins in 30S subunits. These particles, which comprise 50% of the total 30S subunits, are present on polyribosomes. |
| Links | |
| Keywords |
Escherichia coli/genetics; Nucleic Acid Precursors/metabolism; RNA, Bacterial/metabolism; RNA, Ribosomal/metabolism; Ribonucleases/isolation & purification; Ribonucleases/metabolism; Ribosomal Proteins; Transcription, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.