PMID:3549690
| Citation |
Jones, CJ, Homma, M and Macnab, RM (1987) Identification of proteins of the outer (L and P) rings of the flagellar basal body of Escherichia coli. J. Bacteriol. 169:1489-92 |
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| Abstract |
Synthesis of the Salmonella typhimurium hook protein from the gene cloned on a multicopy plasmid results in partial suppression of the flagellar assembly defects of certain classes of Escherichia coli mutants (K. Ohnishi, M. Homma, K. Kutsukake, and T. Iino, J. Bacteriol, 169:1485-1488, 1987). This phenomenon allowed hook-basal body complexes from such mutants to be purified and analyzed by electron microscopy and gel electrophoresis. The absence of the P and L rings in such structures was found to correlate with the absence of proteins of apparent molecular weight 39,000 and 26,000, respectively. Gene-polypeptide correlations from other studies enabled us to complete gene-polypeptide-structure correspondences for these two proteins as flaM----39-kilodalton protein----P ring and flaY----26-kilodalton protein----L ring. |
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| Keywords |
Bacterial Proteins/analysis; Bacterial Proteins/genetics; Cell Movement; Electrophoresis, Polyacrylamide Gel; Escherichia coli/analysis; Escherichia coli/genetics; Escherichia coli/ultrastructure; Flagella/analysis; Flagella/ultrastructure; Genes, Bacterial; Microscopy, Electron; Mutation |
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