PMID:3549459
| Citation |
McCaman, MT and Gabe, JD (1986) The nucleotide sequence of the pepN gene and its over-expression in Escherichia coli. Gene 48:145-53 |
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| Abstract |
The complete nucleotide sequence has been determined for the pepN gene of Escherichia coli K-12. The product of this gene, peptidase N, is apparently 870 amino acids in length. The coding sequence is followed by a tandem pair of stop codons and then a sequence capable of forming a stem-and-loop structure in the pepN mRNA. In the process of subcloning the pepN gene we constructed a plasmid which causes peptidase N to be produced at a level of 50% of total protein. The peptidase is fully active and completely soluble and these overproducing cells appear otherwise normal. |
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| Keywords |
Aminopeptidases/biosynthesis; Aminopeptidases/genetics; Base Sequence; Codon/genetics; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation; Genes; Plasmids; Promoter Regions, Genetic |
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