PMID:3543212
| Citation |
Buck, D, Spencer, ME and Guest, JR (1986) Cloning and expression of the succinyl-CoA synthetase genes of Escherichia coli K12. J. Gen. Microbiol. 132:1753-62 |
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| Abstract |
The genes encoding both subunits of the succinyl-CoA synthetase of Escherichia coli have been identified as distal genes of the suc operon, which also encodes the dehydrogenase (Elo; sucA) and succinyltransferase (E2o; sucB) components of the 2-oxoglutarate dehydrogenase complex. The newly defined genes express polypeptides of 41 kDa (sucC) and 31 kDa (sucD), corresponding to the beta and alpha subunits of succinyl-CoA synthetase, respectively. The genes are thus located at 16.8 min in the E. coli linkage map, together with the citrate synthase (gltA) and succinate dehydrogenase (sdh) genes, in a cluster of nine citric acid cycle genes: gltA-sdhCDAB-sucABCD. Four deletion strains lacking all of these citric acid cycle enzymes were characterized. The succinyl-CoA synthetase activities of strains harbouring plasmids containing the sucC and sucD genes were amplified some fourfold. Further enzymological studies indicated that expression of succinyl-CoA synthetase is coordinately regulated with 2-oxoglutarate dehydrogenase. |
| Links | |
| Keywords |
Cloning, Molecular; Coenzyme A Ligases/genetics; Culture Media; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Expression Regulation; Genes, Bacterial; Glucose/metabolism; Plasmids; Succinate-CoA Ligases/genetics; Succinate-CoA Ligases/metabolism |
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