PMID:3540199
| Citation |
Klein, J, Henrich, B and Plapp, R (1986) Cloning and expression of the pepD gene of Escherichia coli. J. Gen. Microbiol. 132:2337-43 |
|---|---|
| Abstract |
Peptidase D of Escherichia coli, cleaving the unusual dipeptide carnosine, was found to be encoded by the ColE1 hybrid plasmid pLC44-11. From this plasmid the pepD gene was subcloned into small vectors. As shown by successive reduction of the flanking sequences of genomic DNA, the order of genes in the region at 6 min of the E. coli K12 map is phoE, pepD, in the clockwise orientation. Insertional inactivation of the pepD gene and expression of recombinant plasmids in maxicells allowed the identification of the pepD product as a 52 kDa protein. Comparison with the 100 kDa protein molecular mass determined by gel filtration suggests that active peptidase D is probably a dimer. |
| Links | |
| Keywords |
Cloning, Molecular; Dipeptidases/biosynthesis; Dipeptidases/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Plasmids |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.