PMID:3539937
| Citation |
Phillips, GJ and Kushner, SR (1987) Determination of the nucleotide sequence for the exonuclease I structural gene (sbcB) of Escherichia coli K12. J. Biol. Chem. 262:455-9 |
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| Abstract |
The complete nucleotide sequence of the structural gene for Escherichia coli exonuclease I has been determined. The coding region corresponds to a 465-amino acid protein with molecular weight of 53,174. The partial amino acid sequence of purified exonuclease I agrees with that predicted by the DNA sequence. Two putative weak promoters have been localized by S1 nuclease analysis. The sbcB coding sequence contains many non-optimal codons, characteristic of many poorly expressed E. coli genes. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Codon; DNA, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Exodeoxyribonucleases/genetics; Genes; Genes, Bacterial; Molecular Weight; Promoter Regions, Genetic; Transcription, Genetic |
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